Proteomics

You are here

Expression of genes into the ultimate gene product has a central role in the phenotype of an organism. Typically the final product for any gene is a protein than can play a important regulatory, structural, transportation, communication or enzymatic role. All of the proteins and their modifications that are expressed by a an organism is called the proteome. Understanding the expression, localization, functions, interactions, and modifications of proteins within an organism can give insights into the molecular basis of various phenotypes as well as provide a significant dimension to genomic and transciptomic studies. Proteomic services are provided in close collaboration with the Mass spectrometry, Metabolomics & Proteomics Facility (MMPF) at UIC. Data acquired via the state of the art instruments within the MMPF can allow the following types of analyses.

Protein Identification
Bottom up proteomic analysis using high resolution tandem mass spectrometry can allow for the identification of proteins present in biological materials. This technique is well adapted to detecting the presence or absence of proteins under different experimental conditions or treatments of a biological system. Additional downstream analyses, such as gene ontology, pathway analysis or post-translation modification (PTM) analysis, can be used to further elucidate the biological context from proteomic results.
Quantitative Proteomics
Either relative methods, such as iTRAQ, or absolute methods, i.e. SRM/MRM, can be used to provide a quantitative dimension to proteomic results. Furthermore, these techniques can provide a important validation step for any proteomic analysis.